| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060180 |
| Chromosome: | chromosome 1 |
| Location: | 6767451 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048350 | EBM1,CGL53 | (1 of 4) K19355 - mannan endo-1,4-beta-mannosidase (MAN); conserved protein with glycosyl hydrolase family 5 domain | 5'UTR |
| Cre01.g048400 | DZIP1L,DZP1 | DAZ interacting zinc finger protein 1; (1 of 1) K16470 - zinc finger protein DZIP1 (DZIP1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAAATTGACCAAAAGTTCTTCGGAACTTCTGACAGCTCACAACGCTGG |
| Internal bar code: | ATAGCGTCAAAGCGTAGTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4380 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGCCAAAAGCCACAATG |
| Suggested primer 2: | GAGGACTGTGAGGTTGAGCC |