Insertion junction: CLIP2.060182_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.060182
Chromosome:chromosome 3
Location:2887454
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g162500 (1 of 4) IPR011016//IPR013083 - Zinc finger, RING-CH-type // Zinc finger, RING/FYVE/PHD-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCAGACGCCGTCAAGGTATTTAACATTTTAACTTGCCGCCATGGATCT
Internal bar code:ATCAGAATATCTCGTTTGAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1487
LEAP-Seq percent confirming:93.8775
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGGGTGAGGTTTCGTTCG
Suggested primer 2:GTCGAGACAGGGCAATTCCA