| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.060183 |
| Chromosome: | chromosome 8 |
| Location: | 473474 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359051 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACTGCGTCACGGAAAGCGGTTGGCAGGAGCGCCGCACGGGGTGAGCC |
| Internal bar code: | AGCAATGCGAATTCTATAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1499 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCGACAAGCTCTCGGTA |
| Suggested primer 2: | TTTACAGTCCGTGTCGCGAA |