Insertion junction: CLIP2.060191_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.060191
Chromosome:chromosome 1
Location:7858167
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g055420 PP2A1,PP2A-B,PF4 (1 of 1) K04354 - serine/threonine-protein phosphatase 2A regulatory subunit B (PPP2R2); Phosphatase PP2A, B subunit gene_edge/mRNA_edge/5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTAGTCAAACCCCGCGTAGGTAGACTCACCTGGACAGTACTCACGACC
Internal bar code:GTACGCGGGGGTAGAAGTGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:64
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCAAACGATGCTCCACGT
Suggested primer 2:CTTTGTCCAAAGTGCAGGCC