Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.060194 |
Chromosome: | chromosome 5 |
Location: | 2198151 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g237600 | (1 of 1) PF09243 - Mitochondrial small ribosomal subunit Rsm22 (Rsm22) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTAGAGGTAGACGCTTGCGGCCTCAGACTGGATACGTGGCTCAGCCCA |
Internal bar code: | ACCATCCAGATTCGCATGTGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 614 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 11 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGAGGGCATACCATGTTGG |
Suggested primer 2: | CACGGGCTTCATAATTGCGG |