| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.060199 |
| Chromosome: | chromosome 9 |
| Location: | 6387419 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCGCTCCCTAACCAAACACTTACGCACCAGTGGATGGTCTTTCCCACT |
| Internal bar code: | ATCGCCGAGGTTTCTGGCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1230 |
| LEAP-Seq percent confirming: | 90.566 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCCCAAAATTTTCGCGT |
| Suggested primer 2: | CCTTTCCCAAAACTTCGGCG |