Insertion junction: CLIP2.060201_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.060201
Chromosome:chromosome 17
Location:3809675
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g727050 3'UTR
Cre17.g727100 TIC56 (1 of 2) PF14237 - Domain of unknown function (DUF4339) (DUF4339); 56 kDa subunit of translocon at the inner membrane of chloroplasts 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGACAACGTAACCGGCTGCCCTGACGCGGTGTGCGCCGCAAACTGACCA
Internal bar code:TAATTCCGATTCTCGTACTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3498
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGTTTCAGTTGCCTGTG
Suggested primer 2:ATCAGGGCTTCAAGTGGGTG