| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060216 |
| Chromosome: | chromosome 12 |
| Location: | 1715804 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801370 | (1 of 34) IPR011010 - DNA breaking-rejoining enzyme, catalytic core | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGTCTAGCCTTCAAAGGCCCACCCACACACCCACACACCCTACATGC |
| Internal bar code: | GTTGGGTGACCATTGATTGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3524 |
| LEAP-Seq percent confirming: | 68.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCCTAGCCTCTTCCTCT |
| Suggested primer 2: | GCTGAGCACGAGGTATCCTC |