| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060217 |
| Chromosome: | chromosome 7 |
| Location: | 1363799 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322800 | CSA4 | Chlorophyceae-specific gene family A protein | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCAATCTCTTCTACCCTAGCTTTTATCCCCTGCTAGCCCCTGCAGCC |
| Internal bar code: | ATGTAGGACGGGATTGTTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2224 |
| LEAP-Seq percent confirming: | 96.2963 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTACACCCCAGCAGAAGG |
| Suggested primer 2: | GTTGCGCCTGCCATCTATTG |