| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060221 |
| Chromosome: | chromosome 10 |
| Location: | 2773272 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438350 | LTH3 | LAT2 homolog | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCCAGCCAGTAGGTCGGCCCAGTATACCTGCCCCCACTTAAATGGCG |
| Internal bar code: | CATTAAAACCGTAGACACGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5024 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGCGACACATAGGGTT |
| Suggested primer 2: | GCTCATGCTCATGCTCGTTG |