Insertion junction: CLIP2.060223_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.060223
Chromosome:chromosome 9
Location:1586633
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g398900 CWP1,GP1 (1 of 80) IPR003882 - Pistil-specific extensin-like protein; Vegetative cell wall protein gp1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAGAGGAAGACGCCACCACTCGGTCGCCACCAGAATCTGACACGCACC
Internal bar code:AAATAATGATCCGTAACTGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1197
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:AGACTTTGCTGTTTGCTGCG
Suggested primer 2:TTGCAGGCAAAAATGCTCCC