| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060237 |
| Chromosome: | chromosome 12 |
| Location: | 3311152 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498850 | CEP12 | Cysteine endopeptidase; (1 of 3) 3.4.18.1 - Cathepsin X / Lysosomal carboxypeptidase B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGGGCCATGTATCTTATTACCCTGTGCCGGCTTGACGAATACTTATC |
| Internal bar code: | TTAACACGTATTTAATGAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6938 |
| LEAP-Seq percent confirming: | 99.422 |
| LEAP-Seq n confirming: | 172 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 173 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCCACCGGTGTTGTTTGC |
| Suggested primer 2: | AAGTGATTGCTGAGTCGCGA |