Insertion junction: CLIP2.060261_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.060261
Chromosome:chromosome 2
Location:7462219
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g800263 3'UTR
Cre09.g392393 (1 of 781) IPR000104 - Antifreeze protein, type I 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCCCACGTACAGCGGCGACACAGTGCTGCCGGCGCTTTTGTTGTATT
Internal bar code:TGGAGGATCCGGACTGGCGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3122
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CCACAACCTCCTCGTCACTC
Suggested primer 2:CCATTCAGGCCTGTTACCGT