Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.060261 |
Chromosome: | chromosome 2 |
Location: | 7462219 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g800263 | 3'UTR | ||
Cre09.g392393 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCCCACGTACAGCGGCGACACAGTGCTGCCGGCGCTTTTGTTGTATT |
Internal bar code: | TGGAGGATCCGGACTGGCGCTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3122 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 31 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCACAACCTCCTCGTCACTC |
Suggested primer 2: | CCATTCAGGCCTGTTACCGT |