Insertion junction: CLIP2.060271_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060271
Chromosome:chromosome 5
Location:1559588
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g234666 (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase 3'UTR
Cre05.g800535 (1 of 25) IPR001965//IPR011011//IPR013083//IPR019787 - Zinc finger, PHD-type // Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type // Zinc finger, PHD-finger 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTATTGTCTTGTCTGCACGCTAATGATAAAGAGGAAATGCAAAGACCG
Internal bar code:TCTTCCCGAAATGAGATAGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4159
LEAP-Seq percent confirming:12.2807
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTTGTCGGGTGAGGTTTA
Suggested primer 2:AGATTTTCTCGGGCGCTTGA