| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.060285 |
| Chromosome: | chromosome 5 |
| Location: | 1387036 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234639 | CCT4 | T-complex protein 1, delta subunit; (1 of 1) K09496 - T-complex protein 1 subunit delta (CCT4) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTAAGAGACGCGCATCGATCTGACCTTGTCCTCTCTTTCTCTTTCGCT |
| Internal bar code: | TGAGAAGACCAGACCATAGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTACCACAACCGGAGCTT |
| Suggested primer 2: | AAGCTCACGGATTTGGGGAG |