| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.060289 |
| Chromosome: | chromosome 13 |
| Location: | 3716272 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588405 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTATGGTCGTATCCCACCACTCCTGTAACCTCAAGGAGTCCACCTTAG |
| Internal bar code: | ACCACCGCAGGCCCGAGCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1272 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATGTGCCGTTGCTCACC |
| Suggested primer 2: | CATACACCACCAGCCCATGT |