| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.060299 |
| Chromosome: | chromosome 16 |
| Location: | 5742843 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGTTGACACAAAGTCTAGCGCTCTTGTGGCAGCGGCCACCTACCCACT |
| Internal bar code: | TTTCGCTAATCTCATTTGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3156 |
| LEAP-Seq percent confirming: | 38.7755 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTTGTCCTCCCTCTCAT |
| Suggested primer 2: | CGCTAGCCAAACACCCAAAC |