Insertion junction: CLIP2.060300_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.060300
Chromosome:chromosome 17
Location:1762320
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre17.g708800 GSH2 (1 of 1) 6.3.2.3 - Glutathione synthase / GSH synthetase; Glutathione synthetase 5'UTR
Cre17.g708850 (1 of 226) IPR012337 - Ribonuclease H-like domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCAGTATTTTCCTGCATATAGATAGCATCCATGCGCACATTTTCTTTA
Internal bar code:CCGCGTATTTCCGTTGATGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:799
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCAGCAGGTAATTGGGGT
Suggested primer 2:GGAGTACTCGCCAACCACAA