| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060303 |
| Chromosome: | chromosome 4 |
| Location: | 1902231 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218400 | (1 of 9) IPR000210//IPR011042//IPR011333 - BTB/POZ domain // Six-bladed beta-propeller, TolB-like // POZ domain | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGCTCGTGATGATGGTACATGGCTAGGCTGGCGCAGCTGTGTTCGT |
| Internal bar code: | GTGGAGATAAGAGCAAAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1781 |
| LEAP-Seq percent confirming: | 73.5294 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGATTCTCCGAGCTTCC |
| Suggested primer 2: | GCGCGGACATTTTGCCTAAA |