| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.060305 |
| Chromosome: | chromosome 2 |
| Location: | 4982077 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110600 | DEG5 | (1 of 2) PTHR22939:SF99 - PROTEASE DO-LIKE 8, CHLOROPLASTIC; Deg protease | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTACACAAATCGCTGCACTTGGGCCAAGTTGCGAACCTCGCCGCGAT |
| Internal bar code: | TAGCCGTGTAGGTTATCAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1625 |
| LEAP-Seq percent confirming: | 68.75 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACACGATACCGGATCTG |
| Suggested primer 2: | ACGCGACAAGACTGCTGTTA |