| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060305 |
| Chromosome: | chromosome 12 |
| Location: | 3260212 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGAGTAATGATGTCCCGGCGTGGCATGGTAAAGCACGCGTGGGGAAA |
| Internal bar code: | TTGTAGAAGTTCTAAGCGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2475 |
| LEAP-Seq percent confirming: | 98.5714 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAAAAGCGAGCGGTATTG |
| Suggested primer 2: | TGCGTTGTCAGACTTTTGCG |