| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.060313 |
| Chromosome: | chromosome 12 |
| Location: | 8307763 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551050 | (1 of 1) PTHR31802//PTHR31802:SF3 - FAMILY NOT NAMED // 32 KDA HEAT SHOCK PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCCGAAAGATTGACTCGTCTCTGTCAGGAACGGACATTGGCACATCG |
| Internal bar code: | TAGGTTATAACCCCGTGCTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1052 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGTAGAGTGTGCACGAC |
| Suggested primer 2: | ACACACACACACACACGTCT |