| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.060318 |
| Chromosome: | chromosome 2 |
| Location: | 8242483 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143600 | FEE1 | (1 of 1) PTHR12436:SF3 - GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN; Feruloyl esterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGTGCAGGTGACGATGCTGGCGTCCCTCGCGCCCTTCAACTCCTCC |
| Internal bar code: | AGAAAAGTTAGGGCGTCTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATGAGCCTACTCCGTCA |
| Suggested primer 2: | TTGGCCTACTGACACGTGAC |