Insertion junction: CLIP2.060321_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.060321
Chromosome:chromosome 10
Location:2122582
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g433150 (1 of 1) PF01344//PF13415 - Kelch motif (Kelch_1) // Galactose oxidase, central domain (Kelch_3) 3'UTR
Cre10.g433200 UAPA4,UAPA3,XUV3 Xanthine/uracil/vitamin C permease-like; (1 of 4) PTHR11119//PTHR11119:SF36 - XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAACTCCCCACAGAAGGTGCACTGAGCCTTGCACTGAGCTGTTTGTAAC
Internal bar code:TAGGCCGGTTGCTTTTTGCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:269
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTGCAATGCCTGACAAAA
Suggested primer 2:GCACAGCAATGCATAGGAGC