Insertion junction: CLIP2.060325_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.060325
Chromosome:chromosome 4
Location:679802
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAAAACAACATAACACGCCACATTGTATCATACGAGCTTGAGCGTGTAC
Internal bar code:TCGCTCATGTTCAGGCACAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:4167
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:AATGCTGACGAGGCTCAACA
Suggested primer 2:ACGCAGGTGCTTTTTCGTTC