Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.060355 |
Chromosome: | chromosome 11 |
Location: | 313568 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467567 | UBQ1 | Ubiquitin; (1 of 1) PTHR10666:SF150 - POLYUBIQUITIN 3 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGCTGAAGTCGGAGCGCCACACACCCACCTGCACCCAGGCCACCCAC |
Internal bar code: | TTTTTAGGGGCGATGAGAGTCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3935 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 49 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTAGCCGTTTCTGTCACA |
Suggested primer 2: | CGGGAGTGTGAATGGAAGCT |