| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060358 |
| Chromosome: | chromosome 7 |
| Location: | 3869302 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339000 | CrRbcXIIb,RBCX2B | RuBisCO chaperone; (1 of 2) PTHR33791//PTHR33791:SF1 - FAMILY NOT NAMED // CHAPERONIN-LIKE RBCX PROTEIN | 3'UTR |
| Cre07.g339050 | NSG11 | Cofilin/tropomyosin-type actin-binding protein; (1 of 1) K05765 - cofilin (CFL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGGGTTGAGAAGGCCCTCGTCAAGAGCCTGCTTAGCCGTGCGCAGAA |
| Internal bar code: | GATATAGCAAGCGCAGGAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1041 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCACTGGCTGTCTTCTCC |
| Suggested primer 2: | CTGCACCCAAACTGCCATTC |