Insertion junction: CLIP2.060365_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060365
Chromosome:chromosome 5
Location:3505243
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre05.g233550 CBC1 (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCACCCAAGGTTAGGTAGATGGGCGTAACAGGTGCTTCGGGTAAGTAT
Internal bar code:ATTATCAACGGTCTGGCGTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:824
LEAP-Seq percent confirming:14.2857
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTCATGCCACGTGTCATC
Suggested primer 2:TGTGGGAATGGTGTCGAGTG