Insertion junction: CLIP2.060371_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.060371
Chromosome:chromosome 6
Location:5200259
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g283750 HST1 (1 of 1) K12501 - homogentisate solanesyltransferase (HST); Homogentisate solanesyltransferase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAGTCGAGTGCAAGCGAGTGGCCAGGGAAGTCGACCCAGTCGTCCTTA
Internal bar code:GGTTTCACGGAGGGGAAGGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1725
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCGTCATATAAGCACCGA
Suggested primer 2:CGCAATCGCCTGATGTGAAG