| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060377 |
| Chromosome: | chromosome 17 |
| Location: | 4820413 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802100 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCTTCCGCCGTTCCCCACACGTCACACGCCCCTTCAAGTGACCGCAT |
| Internal bar code: | TAGTAGAGTGGAGCTTGTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1423 |
| LEAP-Seq percent confirming: | 80.8511 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGTCCAGCAAGCATCGC |
| Suggested primer 2: | GTGGCTCTCCCTTGCTTCAT |