Insertion junction: CLIP2.060379_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.060379
Chromosome:chromosome 17
Location:2292211
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre17.g713450 HTB31 (1 of 27) K11252 - histone H2B (H2B); Histone H2B CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACATCTACAAGGTGCTCAAGCAGGTGCACCCCGACACCGGCATCAGCAG
Internal bar code:ACAAGGTTTGTAGGCCGTCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2355
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGAACCGCTTCAGTCTGT
Suggested primer 2:GTTGGGTCAAACAACCGCTC