Insertion junction: CLIP2.060394_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.060394
Chromosome:chromosome 1
Location:4583816
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g031550 CSB6 (1 of 12) IPR004843//IPR029052 - Calcineurin-like phosphoesterase domain, apaH type // Metallo-dependent phosphatase-like; Probable transposon-derived protein of Chlamydomonas-Specific family B 5'UTR
Cre01.g031600 (1 of 2) PF05915 - Eukaryotic protein of unknown function (DUF872) (DUF872) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGCAACATTATCCAAACCAACACTGAAGACAGCAGCTCGTGCACTAGG
Internal bar code:GTGGCAGCGATAAAGGAGAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:308
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCTAGAGGCTACCCACCCTT
Suggested primer 2:AATCGAAACAGCCCTGCTCA