Insertion junction: CLIP2.060420_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.060420
Chromosome:chromosome 6
Location:2865821
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g272350 5'UTR
Cre06.g272400 (1 of 1) PTHR11247:SF1 - DOLICHYLDIPHOSPHATASE 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGACAGAGCGCAAGGCTTGCAGTACTTCCATCGTGTCAAGTGGGATCG
Internal bar code:CTTTAGAGAGTCGCAATTATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3645
LEAP-Seq percent confirming:48.8189
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:65
LEAP-Seq n unique pos:127

Suggested primers for confirmation by PCR

Suggested primer 1:GGGATTTGAGTCGATTGCGC
Suggested primer 2:CCGTCGCCGAATCTGACATA