| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.060420 |
| Chromosome: | chromosome 6 |
| Location: | 2865826 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272350 | 5'UTR | ||
| Cre06.g272400 | (1 of 1) PTHR11247:SF1 - DOLICHYLDIPHOSPHATASE 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTAAATGCAGTTATAGCATATGTATTGTAATTAACAGTACCTCGCATT |
| Internal bar code: | ATATCAAATACTGGGCGATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1155 |
| LEAP-Seq percent confirming: | 45.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTCGCCGAATCTGACATA |
| Suggested primer 2: | GGGATTTGAGTCGATTGCGC |