| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060437 |
| Chromosome: | chromosome 16 |
| Location: | 1426683 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652350 | CMA1,FAP183 | Chlamydomonas-specific membrane protein family A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAACCCCATTGCGCATTCGCGCCATGAAGTCTTCATGAGCCACTCATG |
| Internal bar code: | ATGTCTATGAACGCGCGTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1834 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAGACCTCATGGCGCAA |
| Suggested primer 2: | AGCCCGTATTGCGACGATAG |