| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060449 |
| Chromosome: | chromosome 7 |
| Location: | 575919 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316550 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCTGCTTGAGCTTGCATTCATAGCTTGCAGAAGTACTCAACAGTTTGA |
| Internal bar code: | CAGTGTGCGAGTACATTTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3223 |
| LEAP-Seq percent confirming: | 69.0476 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTTTCGTGCCTCCAGTCC |
| Suggested primer 2: | CCCTGTGCGGAAGAAGAGTT |