Insertion junction: CLIP2.060462_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060462
Chromosome:chromosome 12
Location:2849457
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g503200 (1 of 3) 3.4.24.13 - IgA-specific metalloendopeptidase / Immunoglobulin A1 proteinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCACATTCGTACCGGTCGTATCACACGGCCAACCTACCGCGCTAGCTA
Internal bar code:CGTTTGTACCACGTCGTTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2837
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGAGACTGTGCCCAAGTG
Suggested primer 2:AAAGCATTCCGCCATCTCCA