| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060465 |
| Chromosome: | chromosome 9 |
| Location: | 412723 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749647 | (1 of 3) 6.3.4.13 - Phosphoribosylamine--glycine ligase / Phosphoribosylglycinamide synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTTTTACTACCCTCCACTCCAACCTCTACCCTTCTTACCACTCGATGC |
| Internal bar code: | TTTAGGGGTGCCCACGGTCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGTGTACGTGCTGGTGA |
| Suggested primer 2: | GCCCCAGGTTGAGTTGCTAT |