| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.060473 |
| Chromosome: | chromosome 4 |
| Location: | 3802670 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g800498 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGAAGGTATATCATTTGAAATTGAAGGTGGCAAGGAACGCTTGCTTGC |
| Internal bar code: | TGACGCGAGGGATAATCCCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGTGGGTTTGTTAGGTCG |
| Suggested primer 2: | TGGGTCTTGAGCTCCGGATA |