| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060476 |
| Chromosome: | chromosome 9 |
| Location: | 275982 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386759 | (1 of 46) IPR000742 - EGF-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCGAACTCTTGAAGTTCAAACTCTCAACTTTCTTGACGCTCAAAGCA |
| Internal bar code: | ACGCCCAATGATGTTTTCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2155 |
| LEAP-Seq percent confirming: | 87.234 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTCCACACACAGCAGCAG |
| Suggested primer 2: | CCATGCAGCCGTACTAGGAG |