Insertion junction: CLIP2.060478_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060478
Chromosome:chromosome 7
Location:3735875
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATAGATTGCTCATCCCCGACATCGGATAGCTCCAACAGCGCGATGCAGT
Internal bar code:TATACGGATAAGAGTCCTTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2413
LEAP-Seq percent confirming:25.4237
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTGAAGCCTGGAAGACA
Suggested primer 2:CAAACTGACGCGTGAAAGCA