| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060478 |
| Chromosome: | chromosome 10 |
| Location: | 3447364 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801144 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTATCGATAGACTGGTCGTGCTGTATTGCACTAGGCTGGCCATTGGCGG |
| Internal bar code: | GGTGGCGTGCAGTACTGCATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1718 |
| LEAP-Seq percent confirming: | 86.8421 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGCAAGGTTGGTTGGGT |
| Suggested primer 2: | ACCCATACTCCCTCCTCACC |