Insertion junction: CLIP2.060479_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.060479
Chromosome:plastome
Location:69738
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802282 psaA_exon3,ChreCp019,2717000 photosystem I P700 apoprotein A1 transpliced exon 3 of 3; (1 of 1) K02689 - photosystem I P700 chlorophyll a apoprotein A1 (psaA) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAACTTAGCTTTATTTGGTTCGTTATCAATTATTGTAGCTCACCACATG
Internal bar code:GATGTGACCCTGTTTATACAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:176
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGACCGTCACAAGGGAAA
Suggested primer 2:GCTGGTTGGTTCCACTACCA