Insertion junction: CLIP2.060488_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.060488
Chromosome:chromosome 14
Location:2146197
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g621850 (1 of 4) PF13369 - Transglutaminase-like superfamily (Transglut_core2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCTTCAAGCACACGAGAGGGAGCCCACGTGGCGGATTTATTGTGTGGT
Internal bar code:CTACTCAGCCAACCTCGCACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1389
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTTCCCTGTCACACTCAC
Suggested primer 2:CTCGGAACTTCCCAACGGAA