| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060492 |
| Chromosome: | chromosome 1 |
| Location: | 7810658 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g055100 | (1 of 1) IPR000104//IPR011016 - Antifreeze protein, type I // Zinc finger, RING-CH-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTGCCATCTGCTGCTTACCTGCCAAACTACGGCAGCAGATCACGACGG |
| Internal bar code: | TGGTTATGAGCACTTGTGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 10.5263 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGACCAAGACACCCCAG |
| Suggested primer 2: | ATTACACGCACACGCAAACC |