| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.060503 |
| Chromosome: | chromosome 12 |
| Location: | 4549809 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521350 | SLY1 | (1 of 1) PTHR11679:SF2 - SEC1 FAMILY DOMAIN-CONTAINING PROTEIN 1; SM/Sec1-family protein, SLY1 homolog | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCACAAGCGCGCCTCCCAGACTTGCAACGTTGGAAAATAAAGCAAGT |
| Internal bar code: | ACCGTTTTCCGGACGGAAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1550 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCGCAACCTACACCTAC |
| Suggested primer 2: | ACCTCTCCACCTGATACCCC |