| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060534 |
| Chromosome: | chromosome 10 |
| Location: | 3733586 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801158 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTCGCGCTCACCAGGACTGAGCATGTGAATCCCGAAAGCTTCCCGAAT |
| Internal bar code: | TATGGGCATTGGGTATCTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 14.2857 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACTTGCTCGCAATGGGC |
| Suggested primer 2: | CATGTCTCTACATCGGGCCC |