| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.060545 |
| Chromosome: | chromosome 12 |
| Location: | 1455577 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488250 | (1 of 2) K17822 - DCN1-like protein 1/2 (DCUN1D1_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCGGTAGCCCCGCTCCCATCTTCCAACACCACCGCCACCGCCACCTT |
| Internal bar code: | ATTGGGGCTGTAGTTTAGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1038 |
| LEAP-Seq percent confirming: | 64.5161 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCATCACCATCACCAGCAG |
| Suggested primer 2: | CTGGTGCTTGACGTGAGACT |