| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060558 |
| Chromosome: | chromosome 4 |
| Location: | 2076690 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219500 | DNJ30 | (1 of 1) PTHR22904:SF338 - LD24721P; DnaJ-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCGGCACTGCCTGGGCTGGGTGCAAATCATTCCCGGAAAGGTGTGCG |
| Internal bar code: | GCAATGTCTTACTCGCGATCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2454 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCAGAAATTTGCCCCAAA |
| Suggested primer 2: | AAAAGTCTGCTCCCACCGAG |