Insertion junction: CLIP2.060566_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.060566
Chromosome:chromosome 10
Location:92496
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g418150 FAP178 (1 of 1) IPR001715//IPR010441 - Calponin homology domain // CH-like domain in sperm protein; Flagellar Associated Protein 178 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGCCCGGGCTCTCACGAGCGAGAATGTGAACGCTGTTTGCGCGTGGAA
Internal bar code:CGCATATTTTAGGACGATTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:2313
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:TAGACGATGGTGGCGTATGC
Suggested primer 2:GCGCAAACTCAACACATGGT